effects of various activators on bovine embryonic development following intracytoplasmic sperm injection

Effects of Various Activators on Bovine Embryonic Development Following Intracytoplasmic Sperm Injection

The objective of this study was to compare the effectiveness of various combination activators of bovine oocyte following intracytoplasmic sperm injection (ICSI) in terms of cleavage and embryo development. Bovine ICSI oocytes were treated with a variation of treatments of combined or single activators as follows; 30 V for 15 x 2 μsec of electrical pulse +10 μg/mL cycloheximide (CHX),7% ethanol...

Effect of polyvinylpyrrolidone on sperm function and early embryonic development following intracytoplasmic sperm injection in human assisted reproduction

The objective here was to review the effects of polyvinylpyrrolidone (PVP) upon sperm function and embryonic development in humans. PVP has been used successfully in intracytoplasmic sperm injection (ICSI) to facilitate the handling and immobilization of sperm for both domestic animals and humans. In our previous reports, PVP solution exists locally in embryos injected during the early developm...

Effects of Sperm Chromatin Integrity on Fertilization Rate and Embryo Quality Following Intracytoplasmic Sperm Injection

Sperm chromatin integrity has been being recognized as an important factor in male fertility. During normal fertilization, high quality sperm with intact chromatin are selected through natural selection in journey from vagina to fallopian tube. However, using Assisted Reproductive Techniques, particularly ICSI, the natural selection is bypassed. Therefore sperm with DNA breakage have the opport...

effects of sperm chromatin integrity on fertilization rate and embryo quality following intracytoplasmic sperm injection

sperm chromatin integrity has been being recognized as an important factor in male fertility. during normal fertilization, high quality sperm with intact chromatin are selected through natural selection in journey from vagina to fallopian tube. however, using assisted reproductive techniques, particularly icsi, the natural selection is bypassed. therefore sperm with dna breakage have the opport...

Intracytoplasmic sperm injection (ICSI).

Intracytoplasmic Sperm Injection

Intracytoplasmic Sperm Injection (ICSI) is an assisted reproductive technique [4] (ART) initially developed by Dr. Gianpiero D. Palermo [5] in 1993 to treat male infertility [6]. It is most commonly used in conjunction with in vitro [7] fertilization [8] (IVF) or a less commonly used technique called zygote intrafallopian transfer [9] (ZIFT). In natural fertilization [8], the sperm [10] must pe...